Many plant extracts and essential oils have been screened for their nematicidal activity against the plantparasitic nematodes814. In the present study, we evaluated the potential of 5 selected plants to inhibit test bacteria by agar well diffusion assay and the result is shown in table 2. A laboratory exercise on determining the antioxidant activity of plant extracts jacinto et al. Pdf nematicidal activity of plant extracts against the root. Jain5 1department of pharmacognosy, mcops, manipal576104, india. Extraction, isolation and characterization of bioactive compounds. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. Such results were different from data reported in literature. These products are well recognized for their health benefits in many societies around the world. The chemical examination follows after the isolation and biological screening of the active fraction1. Antimicrobial activity of crude extracts of three medicinal. By ngaruiya paul njuguna a thesis submitted in partial fulfilment of master of. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. The experiment confirmed the efficacy of some selected plant extracts as natural antimicrobials and suggested the possibility of employing them in drugs for the treatment of infectious diseases caused by the test organisms.
Phytochemical analysis of phyllanthus amarus extracts s. Screening of in vitro antioxidant activity of methanolic. Concentrationresponse curves for the antiproliferative effect. Antimicrobial activity of selected medicinal plants against. Biochemistry graduates to have results from their final. The aim of this study was to assess the efficacies of three plant extracts for their potential antioxidant and antiinflammatory activity in primary human skin fibroblasts. Antioxidant, antihypertensive, antihyperglycemic, and. Rajendaran1, durga jaiswal2, hari pratap singh3, ashutosh mishra3, dinesh chandra4, indranil kumar yadav5and d. Cold and hot water leaf extracts of 25 plant species comprising horticultural crops including multipurpose trees, weeds, forage crops and medicinal plants at 5% group i and 30 forest tree species at 10% group ii were tested against the brinjal aubergine, solanum melongena damping off pathogen, pythium aphanidermatum in pda cultures. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. In the present study, methanol and aqueous extracts of parkinsonia aculeata l.
The emergence of drug resistant variants of the influenza virus has led to a need to identify novel and effective antiviral agents. These data suggest that the combination of in vitro bioassays for estrogenic activity along with isolation and characterization of the active components by lcms is a valid approach for identification of plant extracts beneficial. Chemical composition and antibacterial activity of. Determining the antioxidant property of plant extracts. Antioxidant activity was studied using dpph, cuprac, reducing power assay, deoxyribose degradation site and nonsite specific, ferric reducing antioxidant potential frap, ferric thiocyanate ftc. Phytochemical investigation and biological activity of. Antimicrobial activity of some plant extracts against. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated.
Pdf of full length paper is available online introduction tea is a gift of. Furthermore, nine of these 15 plant extracts also exhibited greater than 25%50% inhibition of hepatitis bvirus hbv dna polymerase activity, and also significantly stimulated production of. Antioxidant activities of aqueous extracts of selected plants. The focus of this research is the evaluation of the nematicidal activity of water extracts from pokeweed phytolacca americana. The analysis of bioactive compounds present in the plant extracts involving.
Phytochemical investigation and biological activity of leaves. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. The ethanolic extracts had a mixture of compoundsfractions and it is possible that the antiviral activity of plants is decided by one or several compounds or combinations thereof 34. In vitro antibacterial activity of several plant extracts. Plant phenolic extracts as an effective strategy to control.
Avian infectious bronchitis, plant extracts, antiviral activity background ib is a highly contagious respiratory and occasionally urogenital disease in chickens 1. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. The stable radical dpph has been used widely for the determination of primary antioxidant activity, that is, the free radical scavenging activities of pure antioxidant compounds, plant and fruit extracts and food materials. Identification of traditional medicinal plant extracts with. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Antisnake venom activity of different extracts of pouzolzia indica against russel viper venom anas ahmed1, k. Identification of traditional medicinal plant extracts. Antioxidant properties of the methanol extracts of the leaves. Antibacterial activity of plants 719 effective extracts are considered, e. Herbs, spices, plant extracts and essential oils eo have been used for centuries to preserve food for humans and to make it more appetizing. Pdf antiviral activity of some nigerian medicinal plant.
Antioxidant and potential antiinflammatory activity of. Antioxidant activity of plant extracts containing phenolic. Pdf in vitro antiviral activity of plant extracts from. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Evaluation of the cytotoxic activity of extracts from. The antiviral activity of plant extracts against bvdv, hsv1, pv2 and vsv was assessed in vitro by the viral cpe reduction assay. These results show that the aqueous extracts of five native plants of. Biochemistry graduates to have results from their final year. Antimicrobial and antioxidant activity of essential oil and different.
The objective of this study was to determine the chemical composition and antibacterial activity of essential oils eos of tagetes minuta against three phytopathogenic bacteria pseudomonas savastanoi pv. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Debnath department of pharmaceutical sciences, dibrugarh university, dibrugarh 786 004, india. Cytotoxic properties of some medicinal plant extracts from.
The aim of this study was to identify plant extracts with proven. Studies on some plant extracts for their antimicrobial. Extracts of 40 green, black or oolong high quality tea samples from china, japan or india among others, have been prepared according to the manufacturer instructions and assayed for total po lyphenols content using the folinciocalteu phenol reagent. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a.
The concentration of extracts in lard varied from 0. Results of antimicrobial activity of the five plant extracts can suggested that s. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29. Paola diaz dellavalle1, andrea cabrera 1, diego alem 1, patricia larranaga1, fernando ferreira2, and marco dalla rizza1 abstract the aim of the study was to evaluate the antifungal activity of extracts of 10 plant species used in traditional uruguayan.
Ethanol extracts were evaluated for cytoxicity by using mtt assay on llcmk2 cells and by brine shrimp lethality assay. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether. Plant extracts 1 ml were allowed to react with 1 ml of the abts solution and the absorbance was taken at 734 nm after 7 min using the spectrophotometer. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. One such alternative tactic is the use of plant extracts. In vitro antiviral activity of plant extracts from. Thus, the present study aims to assess the antibacterial activity of several methanol. Ten dif ferent plants were collected from in and around sivakasi area. Experimental, in vitro, evaluation of the activities of 28 plant extracts and oils as well as some antibiotics against e.
In order to extract different phenolic compounds from plants with a high degree. As an alternative to synthetic drugs, the consolidation of empirical knowledge with ethnopharmacological evidence of medicinal plants offers a novel platform for the development of antiviral drugs. Antioxidant properties of the methanol extracts of the. Antimicrobial activity of ethanol leaf extracts of. Antifungal activity of medicinal plant extracts against phytopathogenic fungus alternaria spp.
Evaluation of estrogenic activity of plant extracts for. A comparative evaluation of antifungal activity of. Antifeedant activity of plant extracts to an insect helopeltis theivora a. Antioxidant activity of some plant extracts of the family. Antiviral activity of some nigerian medicinal plant extracts. A comparative evaluation of antifungal activity of medicinal. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Anurag sharma and rakesh gupta abstract in the present study, aqueous extract of eight plants, namely azadirachta indica a. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Fresh leaves of eleven different plant species free from.
Many extracts of plants acted against ibv prior to and d uring infection, but the most effective were those of m. For measuring radical scavenging activity rsa against the stable radical n,n diphenylnpicrylhydrazyl dpph, 0. The leaf extracts of romatic plants, murraya koenigii and azadirachta a. Antibacterial activities of some borneo plant extracts. The antimicrobial activities of methanolic extracts of. Antibacterial activity of crude extracts of selected plants. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material.
Antimicrobial activity of crude extracts of some plant leaves. The present study aimed to assess the cytotoxic activity of extracts of selected plant species used for treatment of malaria in tanzania. Pdf antibacterial activity of medicinal plant extracts. Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibioticresistant bacteria. Antioxidant activity of plant extracts containing phenolic compounds. Antibacterial activity of aqueous extract of calotropis. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. Antibacterial activity of plant extracts and phytochemicals. Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita. The antiviral activity of the most active extracts was characterized by evaluating their effect on the pretreatment, the virucidal activity and the effect on the adsorption or postadsorption. The results revealed that the extracts of the two plant species caused a considerable reduction in the number of weevils. Increasingly, natural products have potential for the treatment of cancer. Antimicrobial activity of selected medicinal plants.
Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants. In the course of our screening studies for the anticancer compounds from plants, we performed the present study to evaluate the in vitro cytotoxic activity of four plant extracts that we gathered from north of iran, mazandaran, by using human cervix carcinoma cell line, hela. The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated as abts radical scavenging activity % abs control. Evaluation of estrogenic activity of plant extracts for the. Pdf nematicidal activity of plant extracts against the. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. Biological screening of various medicinal plant extracts.
Biochemistry graduates to have results from their final year research projects on the antibacterial activity of plant extracts submitted for publication dilruba meah and aiysha thompson have just finished their biochemistry. Review cytotoxic studies of plant extracts cytotoxicity of plant extracts on both plant and animal test material is capturing scientific interest, as it can indicate their antitumor and anticarcinogenic potentials, which have always been a gateway for the development of novel anticancer drugs. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. In vitro antibacterial activity of several plant extracts and. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Medicinal plants, ethanol, methanol and aqueous extracts, human pathogens, antimicrobial activity. Biological activity of some plant extracts against pieris. Materials and methods plant species and sample preparation. It is difficult to distinguish between the different eo available, as each eo is. The assay is based on the reduction of dpph radicals in methanol which causes an absorbance drop at 515 nm. In nonsite specific degradation assay, methanol and aqueous extracts show the inhibition of 71.
Comparative analysis of the antioxidant and freeradical. Evaluation of nematicidal activity of ethanol extracts of. The activity against 15 isolates of each bacterium was determined by disc diffusion method at a concentration of 5%. Results obtained from this screening table 2 showed that b.
Nematicidal activity of plant extracts against the rootknot nematode, meloidogyne incognita article pdf available in the open natural products journal 21 october 2009 with 2,550 reads. In vitro antiviral activity of fifteen plant extracts. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. Ageratina adenophora also showed antifungal activity against rhizopus spp. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. Chemical composition and antibacterial activity of essential. Biological activity of some plant extracts against pieris brassicae linn. Screening of in vitro antioxidant activity of methanolic leaf. Assessment of antioxidant activity of plant extracts. In vitro antimicrobial activity of some medicinal plants. In this study, the majority of plant extracts were found to have some antiviral activity and could inhibit ibv prior to and during infection. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31.
Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms jianghua liu, joanna e. Juss were evaluated for their growth, adult mortality and oviposition inhibition of c. Screening of antibacterial activity of the medicinal plant. Antioxidant activities of aqueous extracts of selected. Antitumor and antiviral activity of colombian medicinal plant extracts. Assessment of antioxidant activity of plant extracts by. The phytochemical analysis of the crude extracts of the medicinal plants revealed the presence of saponin. Nov 05, 20 in the course of our screening studies for the anticancer compounds from plants, we performed the present study to evaluate the in vitro cytotoxic activity of four plant extracts that we gathered from north of iran, mazandaran, by using human cervix carcinoma cell line, hela. Antifeedant activity of plant extracts to an insect. The scientific world journal hindawi publishing corporation. In searching for natural products as potential antiviral agents, several medicinal plants from the south american folk medicine showed in vitro antiviral activity 24.
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